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Pre-tRNAi Met lacking m1A58 is polyadenylated in trm6-504/ mtr4-20. (Lanes 4–6) Total RNA (5.0 μg) or (lanes 1–3) poly(A)+ RNA (2.0 μg) isolated from the indicated strains cultured at 30°C was separated by denaturing polyacrylamide gel electrophoresis and transferred to a membrane. Hybridization was performed with radiolabeled probe JA11 (tRNAi Met), and tRNAi Met was visualized by autoradiography.
