FIGURE 2.
Deacylation rates of tRNA_12 (tRNAMet CAU) and tRNA_34 (tRNAIle XAU) from H. marismortui. Total RNA was isolated under acidic conditions and subjected to deacylation by base treatment as described in Materials and Methods followed by acid urea PAGE/Northern analysis using probes specific for tRNA_12 (A) and tRNA_34 (B). (aa-tRNA) aminoacyl-tRNA. (C) Comparison of deacylation rates obtained for tRNA_12 (closed squares), tRNA_34 (closed circles), initiator tRNAi Met (open squares), and major isoleucine tRNAIle GAU (open circles).