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. 2008 Jan;14(1):117–126. doi: 10.1261/rna.795508

FIGURE 7.

FIGURE 7.

The post-transcriptional modification present at position 34 in archaeal minor isoleucine tRNAs is not lysidine. In vitro biotinylation of free α-NH2 groups present in modified nucleosides in minor isoleucine tRNAs from E. coli and H. marismortui followed by acid urea PAGE/Northern analysis. (A) T7 transcript corresponding to tRNAIle LAU from E. coli. (Lanes 1,2) Unmodified T7 transcript; (lanes 3,4) T7 transcript after in vitro modification with lysidine using TilS. (B) Analysis of minor tRNAIle in total RNA from E. coli and H. marismortui. (Lanes 1,2) tRNAIle LAU from E. coli; (lanes 3,4) tRNA_34 (tRNAIle XAU) from H. marismortui. Comparison of tRNAs before (lanes 1,3) and after (lanes 2,4) in vitro biotinylation.