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. 2008 Jan;14(1):11–24. doi: 10.1261/rna.774008

FIGURE 5.

FIGURE 5.

Cross-linking from Domain 5. (A) The trans-hydrolysis system for monitoring the association of Domain 5 with an RNA containing the 5′ exon and domains 1, 2, and 3 (exD123) in a reaction that emulates the first step of splicing. (B) D5 oligonucleotides were synthesized with photo cross-linkable analogs s4U (circle) and s6dG (box) at the indicated positions. (C) 5′ 32P-labeled D5 oligonucleotides were preincubated with exD123 under trans-hydrolysis reaction conditions before exposure to 366 nm light. Mg++-dependent cross-links were observed for both D5 molecules containing cross-linkable analogs (lanes 1,3), but not for a wild type D5 (lane 5).