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. 2007 Dec 21;3(12):e192. doi: 10.1371/journal.ppat.0030192

Figure 4. CEL-III Expression in Midguts of Transgenic Mosquitoes.

Figure 4

Transgenic (CEL-III) and non-transgenic (WT) mosquitoes were allowed to feed on naïve mice. After 6 or 24 h, midguts of engorged mosquitoes were dissected and lysed, then electrophoresed on 8% SDS-PAGE. As a control, midguts of sugar-fed mosquitoes (S) were dissected and lysed. CEL-III expression level was examined by western blotting using mouse anti-CEL-III antiserum. Each lane contains protein lysates equivalent to two midguts. The source of protein is indicated at the top of each lane (6, 24 h). For quantitative estimation of CEL-III per midgut, native CEL-III isolated from C. echinata body fluid was analyzed by western blotting. The amount of native CEL-III (5, 25, 100 ng) is indicated at the top of each lane. Arrows indicate the positions of monomeric and oligomeric forms of CEL-III.