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. 2007 Dec 21;3(12):e192. doi: 10.1371/journal.ppat.0030192

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© 2007 Yoshida et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mosquitoes were allowed to feed on a human volunteer. Representative photomicrographs of engorged mosquito gut sections 24 h after a blood meal are shown (HE staining, ×40 magnification for [A and B], and ×1,000 magnification for [C and D]). Midgut of non-transgenic mosquitoes was filled with intact erythrocytes (A and C), with many spaces between erythrocytes. In contrast, no space is observed in the midgut of transgenic mosquitoes (B and D). Erythrocytes appear to be completely hemolyzed, and HE-stained lymphocytes cells are detectable (arrows). Scale bars in (A and B) and (C and D) are 500 μm and 10 μm, respectively.