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. 2007 Dec 21;3(12):e232. doi: 10.1371/journal.pgen.0030232

Figure 8. Deletion of Both Hoxd8 and Hoxd9 Leads to Epithelial Microcyst Formation.

Figure 8

(A) Scheme showing the intact HoxD cluster (top) and two newly produced mouse strains used to characterize Hoxd genes responsible for microcyst formation. The strains are referred to as Del(i-9) or Dup(i-9) for a deletion or a duplication, respectively, and they span from ‘region i' to Hoxd9, inclusively (dashed lined).

(B, C) Kidney cryosections of WT (+/+), Del(i-9) homozygous (Del(i-9)/Del(i-9), Del(1–13) heterozygous (Del(1–13)/+) and Del(1–13)/Dup(i-9) compound mice were either stained with H&E (top), or processed for the detection of Gdf5 (left) or Hoxd9 (right) by ISH. The Del(1–13)/Dup(i-9) compound strain was obtained by breeding Del(1–13) and Dup(i-9) heterozygous mice.

(D) qPCR was used to compare the expression levels of Hoxd9, Hoxd8, Gdf5 and c-myc in newborn kidneys from the mouse strains described before. Values represent the mean of three independent experiments, after normalization to the WT. Independent Student's t test: **, P < 0.01.