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. 2007 Dec 21;3(12):e237. doi: 10.1371/journal.pgen.0030237

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© 2007 Linardopoulou et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Pyrene-actin polymerization assays were conducted with the concentrations indicated for WASH VCA and N-WASP VCA (A). Similar to N-WASP VCA (B), WASH VCA (C) does not promote actin nucleation in the absence of Arp2/3. In the presence of Arp2/3, WASH promotes actin nucleation in a concentration-dependent manner (C).