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. 2007 Dec 21;3(12):e237. doi: 10.1371/journal.pgen.0030237

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© 2007 Linardopoulou et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) A schematic of the washout coding region showing the original P-element insertion allele (wash ^EY15549) and a precise (wash ^exc15) and imprecise (wash ^Δ185) excision allele. Sequence analysis of wash ^Δ185 reveals two stop codons at positions 11 and 12 due to the internal deletion of 1,029 nucleotides and insertion of six nucleotides (underlined) at the junction.

(B) Genetic analysis of washout alleles. No flies homozygous for the wash ^Δ185 excision allele were recovered. CyO is a balancer chromosome that is homozygous lethal.

(C) The wash ^Δ185 mutant phenotype. Bright-field micrograph of pupae heterozygous (left) or homozygous (right) for the wash ^Δ185 allele. The homozygous mutant displays an elongated phenotype and spiracle-eversion (arrows) defects, while the heterozygote is phenotypically normal.