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. Author manuscript; available in PMC: 2007 Dec 20.
Published in final edited form as: Methods Enzymol. 2000;324:200–207. doi: 10.1016/s0076-6879(00)24232-8

Fig. 3.

Fig. 3

BCKDH activity of reconstituted complexes with different ratios of wild-type and mutant E1. A constant amount of purified native E2 (0.75 μg) was used for reconstitution with a constant total amount of E1 (8 μg) but with various ratios of mutant and wild-type E1 (■) Amount of wild-type E1 varied as indicated without addition of mutant E1; (○) amount of total E1 kept constant (8 μg) by varying R288A and wild-type E1; (●) by varying H292A and wild-type E1; (□) by varying D296A and wild-type E1. [Reproduced with permission from J. W. Hawes, R. J. Schnepf, A. E. Jenkins, Y. Shimomura, K. M. Popov, and R. A. Harris. J. Biol. Chem. 270, 31071 (1995).]