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. Author manuscript; available in PMC: 2007 Dec 20.
Published in final edited form as: Methods Enzymol. 2000;324:200–207. doi: 10.1016/s0076-6879(00)24232-8

TABLE I.

Kinetic Parameters of Wild-Type and Mutant E1 Proteinsa

Recombinant E1 Km (α-ketisovalerate) (μM) Vmax (U/mg protein)b
Wild-type 45 ± 13c 5.4 ± 0.7
S293E — —
S293A 532 ± 98 5.5 ± 0.6
S303E 56 ± 9 5.2 ± 0.2
S303A 58 ± 15 5.8 ± 0.7
a

Reproduced in part with permission from Y. Zhao, J. W. Hawes, K. M. Popov, J. Jaskiewicz, Y. Shimomura, D. W. Crabb, and R. A. Harris, J. Biol. Chem. 269, 18583 (1994).

b

Refers to units (μmoles of NADH produced per minute) per milligram of recombinant E1 protein.

c

Mean ± SE; data obtained with four concentrations of α-ketoisovalerate spanning the Km value.