Table 1.
Analysis of the Tryptic Peptide Profiles of 11 Major Exosomal Proteins by MALDI-TOF-MS
| Band | Total no. of peptides | Protein identified (NCBI accession no.) | Matching peptides | Estimated protein molecular mass | Percentage of the protein covered |
|---|---|---|---|---|---|
| kD | % | ||||
| 1 | 15 | Mac-1 α chain (X07640) | 11 | 127.5 | 12 |
| 2 | 20 | n.i. | |||
| 3 | 19 | mP47 = MFG-E8 (Y11684) | 11 | 45 | 24 |
| 4 | 28 | hsc73 (U73744) + | 11 | 70.9 | 20 |
| mP47 = MFG-E8 (Y11684) | 10 | 45 | 23 | ||
| 5 | 15 | mP47 = MFG-E8 (Y11684) | 12 | 45 | 29 |
| 6 | 7 | actin (M12481) | 7 | 41.7 | 20 |
| 7 | 11 | G protein Gi2α subunit (P08752) | 9 | 40.1 | 32 |
| 8 | 9 | calpactin I heavy chain = annexin II (M14044) | 7 | 38.7 | 24 |
| 9 | 9 | MRV provirus gag (core shell protein p30) (S80082) + | 4 | 60.4 | 16 |
| MHC II β chain (P14483) | 4 | 30.1 | 22 | ||
| 10 | 9 | CD9 (P40240) + nonidentified | 4 | 25.2 | 11 |
| 11 | 10 | nonidentified |