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. 1999 Nov 1;147(3):683–693. doi: 10.1083/jcb.147.3.683

Figure 2.

Figure 2

Analysis of the Ipa proteins associated with RBCs membranes after contact hemolysis. RBCs membranes exposed to contact with various strains under different condition were isolated and examined for their Ipa protein content using SDS-PAGE and immunoblotting. Blots were revealed with the H16 anti-IpaB mAb, a mixture of K24, N9, H10, and J22 anti-IpaC mAbs or the 20G9 anti-IpgD and 322F7 anti-IpaA mAbs. The occurrence of contact hemolysis is indicated under each panel. (A) Ipa/Ipg protein associated with RBCs membranes after contact hemolysis with wild-type, ipaC, or mxiD strains at 37°C or 4°C. The blot probed with the anti-IpaB antibody was overexposed to visualize the small amount of IpaB associated with RBC membranes lysed by the ipaC mutant. (B) Association of IpaB and IpaC with RBC membranes isolated after contact with wild-type bacteria at 37°C and stripped, or not (mock), with 5 M NaCl (NaCl), 0.2 M carbonate, pH 11 (carbonate), and 8 M urea (urea). (C) Analysis of the association of IpaB, IpaC, IpgD, and IpaA with RBCs membranes brought into contact with various mutants.