Table 1.
Stable Transformation Assay
| Vectors | HygR | Foci in 10% CS | Foci in 0.5% CS | Soft agar colonies |
|---|---|---|---|---|
| Control | 1,190 ± 137 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| Ras | 1,217 ± 93 | 1,598 ± 56 | 1,472 ± 83 | 1,481 ± 29 |
| IL2Rβ1 | 1,053 ± 64 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| Ras+IL2Rβ1 | 1,095 ± 80 | 1,417 ± 15 | 1,338 ± 55 | 1,325 ± 40 |
| CD2FAK | 1,137 ± 129 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| Ras+CD2FAK | 1,213 ± 110 | 1,080 ± 246 | 1,078 ± 314 | 1,030 ± 320 |
NIH 3T3 cells were cotransfected with RSVHyg and combinations of the plasmids for Ras G12V, IL2Rβ1, CD2FAK, or empty control vector. Total DNA was kept constant. Cells were trypsinized 2 d after transfection and replated in media containing 10% CS with 200 μg/ml hygromycin B, 10% CS, 0.5% CS, or soft agar to measure foci and colony formation. Values are means ± SD from three separate experiments representing the total number of foci or colonies calculated for the entire transfection.