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. 1984 Jan;157(1):73–78. doi: 10.1128/jb.157.1.73-78.1984

Nitrogen fixation and nitrogenase activities in members of the family Rhodospirillaceae.

M Madigan, S S Cox, R A Stegeman
PMCID: PMC215131  PMID: 6581158

Abstract

Strains of all 18 species of the family Rhodospirillaceae (nonsulfur photosynthetic bacteria) were studied for their comparative nitrogen-fixing abilities. All species, with the exception of Rhodocyclus purpureus, were capable of growth with N2 as the sole nitrogen source under photosynthetic (anaerobic) conditions. Most rapid growth on N2 was observed in strains of Rhodopseudomonas capsulata. Within the genus Rhodopseudomonas, the species R. capsulata, R. sphaeroides, R. viridis, R. gelatinosa, and R. blastica consistently showed the highest in vivo nitrogenase rates (with the acetylene reduction technique); nitrogenase rates in other species of Rhodopseudomonas and in most species of Rhodospirillum were notably lower. Chemotrophic (dark microaerobic) nitrogen fixation occurred in all species with the exception of one strain of Rhodospirillum fulvum; oxygen requirements for dark N2 fixation varied considerably among species and even within strains of the same species. We conclude that the capacity to fix molecular nitrogen is virtually universal among members of the Rhodospirillaceae but that the efficacy of the process varies considerably among species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arp D. J., Zumft W. G. Overproduction of nitrogenase by nitrogen-limited cultures of Rhodopseudomonas palustris. J Bacteriol. 1983 Mar;153(3):1322–1330. doi: 10.1128/jb.153.3.1322-1330.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CLAYTON R. K. Photosynthesis and respiration in Rhodospirillum rubrum. Arch Mikrobiol. 1955;22(2):180–194. doi: 10.1007/BF00409304. [DOI] [PubMed] [Google Scholar]
  3. Finke L. R., Seeley H. W. Nitrogen fixation (acetylene reduction) by epiphytes of freshwater macrophytes. Appl Environ Microbiol. 1978 Jul;36(1):129–138. doi: 10.1128/aem.36.1.129-138.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gest H., Kamen M. D. Photoproduction of Molecular Hydrogen by Rhodospirillum rubrum. Science. 1949 Jun 3;109(2840):558–559. doi: 10.1126/science.109.2840.558. [DOI] [PubMed] [Google Scholar]
  5. Habte M., Alexander M. Nitrogen fixation by photosynthetic bacteria in lowland rice culture. Appl Environ Microbiol. 1980 Feb;39(2):342–347. doi: 10.1128/aem.39.2.342-347.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hochman A., Burris R. H. Effect of oxygen on acetylene reduction by photosynthetic bacteria. J Bacteriol. 1981 Aug;147(2):492–499. doi: 10.1128/jb.147.2.492-499.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Howard K. S., Hales B. J., Socolofsky M. D. Nitrogen fixation and ammonia switch-off in the photosynthetic bacterium Rhodopseudomonas viridis. J Bacteriol. 1983 Jul;155(1):107–112. doi: 10.1128/jb.155.1.107-112.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kamen M. D., Gest H. Evidence for a Nitrogenase System in the Photosynthetic Bacterium Rhodospirillum rubrum. Science. 1949 Jun 3;109(2840):560–560. doi: 10.1126/science.109.2840.560. [DOI] [PubMed] [Google Scholar]
  9. LINDSTROM E. S., LEWIS S. M., PINSKY M. J. Nitrogen fixation and hydrogenase in various bacterial species. J Bacteriol. 1951 Apr;61(4):481–487. doi: 10.1128/jb.61.4.481-487.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LINDSTROM E. S., TOVE S. R., WILSON P. W. Nitrogen fixation by the green and purple sulfur bacteria. Science. 1950 Aug 18;112(2903):197–198. doi: 10.1126/science.112.2903.197. [DOI] [PubMed] [Google Scholar]
  11. Madigan M. T., Wall J. D., Gest H. Dark anaerobic dinitrogen fixation by a photosynthetic microorganism. Science. 1979 Jun 29;204(4400):1429–1430. doi: 10.1126/science.204.4400.1429. [DOI] [PubMed] [Google Scholar]
  12. Masters R. A., Madigan M. Nitrogen metabolism in the phototrophic bacteria Rhodocyclus purpureus and Rhodospirillum tenue. J Bacteriol. 1983 Jul;155(1):222–227. doi: 10.1128/jb.155.1.222-227.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Meyer J., Kelley B. C., Vignais P. M. Aerobic nitrogen fixation by Rhodopseudomonas capsulata. FEBS Lett. 1978 Jan 15;85(2):224–228. doi: 10.1016/0014-5793(78)80460-8. [DOI] [PubMed] [Google Scholar]
  14. Meyer J., Kelley B. C., Vignais P. M. Nitrogen fixation and hydrogen metabolism in photosynthetic bacteria. Biochimie. 1978;60(3):245–260. doi: 10.1016/s0300-9084(78)80821-9. [DOI] [PubMed] [Google Scholar]
  15. Stewart W. D. Nitrogen fixation by photosynthetic microorganisms. Annu Rev Microbiol. 1973;27:283–316. doi: 10.1146/annurev.mi.27.100173.001435. [DOI] [PubMed] [Google Scholar]
  16. van Gemerden H. Growth measurements of Chromatium cultures. Arch Mikrobiol. 1968;64(2):103–110. doi: 10.1007/BF00406968. [DOI] [PubMed] [Google Scholar]

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