Table 1.
Catalytic Activity of BbPDF and LpPDF Variantsa
| enzyme | metal content | kcat (s−1) | KM (μM) | kcat/KM (M−1s−1) | ||
|---|---|---|---|---|---|---|
| Zn | Co | Fe | ||||
| Zn-BbPDF1-HT | 1.0 | 0 | 0 | 5.4 ± 0.1
20 ± 1b |
2.5 ± 0.2
510 ± 70b |
2.2 × 106
3.9 × 104b |
| Co-BbPDF1-HT | 0.02 | 1.0 | 0 | 10 ± 1
26 ± 1b |
3.9 ± 0.7
580 ± 60b |
2.6 × 106
4.5 × 104b |
| Fe-BbPDF1-HT | 0.08 | 0 | 0.59 | 3.8 ± 0.1 | 1.9 ± 0.2 | 2.0 × 106 |
| Zn-BbPDF2-HT | ND | ND | ND | 5.3 ± 0.5 | 2.8 ± 1.0 | 2.0 × 106 |
| Zn-BbPDF3-HT | ND | ND | ND | 0.062 ± 0.011 | 6.3 ± 1.2 | 1.0 × 104 |
| Zn-BbPDF2 | ND | ND | ND | 5.6 ± 0.9 | 2.3 ± 0.6 | 2.5 × 106 |
| Native BbPDF | 1.1 | 0 | 0 | 4.5 ± 1.2 | 3.3 ± 0.4 | 1.4 × 106 |
| Zn-LpPDF-HT | ND | ND | ND | 0.9 ± 0.1 | 17 ± 0 | 5.4 × 104 |
| Co-LpPDF-HT | ND | ND | ND | 5.8 ± 0.3 | 22 ± 1 | 2.6 × 105 |
| Fe-LpPDF-HT | ND | ND | ND | 4.3 ± 0.1 | 15 ± 1 | 3.0 × 105 |
a
Unless otherwise noted, the activities reported were determined with f-ML-pNA as substrate;
b
activity toward f-MAS; ND, not determined.