Figure 2.

Effect of MnCl₂ on NO_2– production and luteinizing hormone-releasing hormone (LHRH) release from the same tissues Open bars represent basal NO₂⁻ and LHRH released into the medium. Filled or hatched bars represent NO₂⁻ and LHRH released following stimulation by MnCl₂ at 50 and 250 μm, respectively. Note that 50 μm MnCl₂ did not increase NO₂⁻ (A), but markedly induced LHRH release (B). Similar effects were observed with 100 μm MnCl₂ (not shown). The 250 μm MnCl₂ dose caused increases in both NO₂⁻ (C) and LHRH (D). Together, these data suggest that MnCl₂ can only stimulate NO production at higher concentrations. Each bar represents the mean (± s.e.m.). The number of tissues per group is depicted within each panel. ***P < 0.001 versus respective medium. n = 9 for A and B, and n = 14 for C and D.