Figure 2.
siRNA directed against STIM1 inhibits agonist-activated ARC channel activity A, the effects of siRNA expression on mean inward currents activated by low (0.5 μm) carbachol concentrations. Mean ±s.e.m. carbachol-activated inward current density at −80 mV in cells expressing control siRNA (black, n = 9) are compared with those in cells expressing the siRNA targeted to STIM1 (red, n = 12). B, representative trace showing the activation of inward current at −80 mV following addition of 0.5 μm carbachol (CCh) to a siRNA-expressing cell. Subsequent addition of arachidonic acid (8 μm) failed to further increase the carbachol-activated current, which was fully blocked by La3+ (100 μm).