Figure 1.

(A) C12 antibody recognizes a single 106-kDa polypeptide on Western blot. A 50 μg A31 whole cell extract was separated on 10% polyacrylamide/SDS gel, then transferred to polyvinylidene difluoride membrane and hybridized with C12 antibody. (B) In vitro-translated MAH has 106-kDa molecular weight. In vitro-translated proteins were separated with 10% polyacrylamide/SDS gel electrophoresis and examined by autoradiography. Lanes: 1, luciferase (positive control); 2, no DNA in the reaction (negative control); 3, in vitro-translated product of the full length MAH cDNA. (C and D) Analysis of protein fractions collected from C12 affinity column by silver staining (C) and Western blot (D).