Figure 4.

Colocalization of MAH with splicing factors. Fixed and permeabilized HeLa cells were stained with C12 (a), anti-SC35 (a′), or costained with C12 and anti-SC35 (a") primary antibodies. Then C12 antibody was detected with rhodamine conjugated secondary antibody (a and a"), whereas an FITC-conjugated secondary antibody was used to visualize anti-SC35 (a′ and a") antibodies. The staining pattern was examined with a confocal laser beam microscope.