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Effect of the introduction of the blaOXA-66 gene from A. baumannii into E. coli TOP10 cells on imipenem susceptibility. (A) TOP10 only. (B) TOP/pBAD/Myc-His. (C) TOP10/pBAD/Myc-His-oxa66-1254. (D) TOP/pBAD/Myc-His-oxa66-1266. The imipenem susceptibility tests were determined by Etest. The expression of OXA-66 carbapenemase was induced by the addition of 0.002% arabinose to the agar plate. The MICs are indicated by arrows.
