TABLE 2.
Characteristics of the sequence of the IQ element from strain Spn529a
| Coordinates (bp) | ORF | Description and homology |
|---|---|---|
| 15,434-16,579 | tnp1 | Domain structure similarity with transposase proteins belonging to the ISL3 family (74.2% homology with ISSha1 of Staphylococcus haemolyticus, DDBJ accession no. AP006716) |
| 16,940-17,359 | hol | Belonging to phage holin family proteins (70.1% homology with the corresponding sequence of Dehalococcoides ethenogenes, REFSEQ accession no. YP_181786) |
| 17,361-17,930 | nf3 | Structure homology with N-acetylmuramoyl-l-alanine amidase family proteins (76.0% homology with the corresponding sequence of Bacillus cereus ATCC 10987, GenBank accession no. AE017194) |
| 18,715-20,267 | rec1 | Putative DNA recombinase (90.1% homology with the corresponding sequence of B. cereus ATCC 10987) |
| 20,231-20,680 | nf5 | Hypothetical protein (92.8% homology with the corresponding sequence of B. cereus ATCC 10987) |
| 20,684-22,255 | rec2 | Putative DNA recombinase (92.2% homology with the corresponding sequence of B. cereus ATCC 10987) |
| 22,687-23,904 | mef(I) | Homologies: 93.6% with mef(E) of the mega element (GenBank accession no. AF274302), 91.4% with mef(A) of Tn1207.1 (GenBank accession no. AF227520), 92.3% with a mef variant detected in S. pyogenes in Norway (GenBank accession no. DQ016305), and 92.1% with a mef variant of group G streptococci (EMBL accession no. AJ617704) |
| 24,019-25,482 | msr(D) | Homologies: 96.2% with msr(D) (mel) of the mega element, 95.9% with msr(D) (orf5) of Tn1207.1, and 93.0% with an msr(D) variant of group G streptococci (EMBL accession no. AM084232) |
| 25,614-26,081 | nf9 | Hypothetical protein |
| 26,199-26,780 | nf10 | Domain structure similarity with oxidoreductase family proteins |
| 26,767-27,267 | nf11 | Hypothetical protein belonging to an uncharacterized protein family (UPF0157) (92.4% homology with the corresponding sequence of Bacteroides ovatus, EMBL accession no. AJ557257) |
| 27,589-28,248 | catQ | Chloramphenicol acetyltransferase (96.5% homology with the corresponding catQ gene of Clostridium perfringens, GenBank accession no. M55620) |
| 28,952-30,097 | tnp1 | Domain structure similarity with transposase proteins of the ISL3 family |
a
The IQ element (15,115 bp) forms the right portion of the 5216IQ complex (accession no. AJ971089).