TABLE 2.

Results of single-step resistance selection studies

Strain; description	Drug	Mutation frequency at:
		2× MIC	4× MIC	8× MIC
SA510; MRSA, VRSA	CG400549	∼6.8 × 10−9	∼3.4 × 10−9	∼6.8 × 10−9
	Vancomycin	NTa	NT	NT
	Linezolid	<3.3 × 10−10	<3.3 × 10−10	<3.3 × 10−10
	Quinupristin-dalfopristin	∼3.3 × 10−6	∼3.3 × 10−8	<3.3 × 10−10
	Daptomycin	∼2.6 × 10−7	<2.6 × 10−10	<2.6 × 10−10
SA509; MRSA, VRSA	CG400549	∼3.0 × 10−7	∼1.5 × 10−8	∼1.5 × 10−8
	Vancomycin	NT	NT	NT
	Linezolid	<2.6 × 10−10	<2.6 × 10−10	<2.6 × 10−10
	Quinupristin-dalfopristin	∼3.9 × 10−9	<2.6 × 10−10	<2.6 × 10−10
	Daptomycin	<1.7 × 10−10	<1.7 × 10−10	<1.7 × 10−10
SA555; MRSA, VISA	CG400549	∼2.0 × 10−9	<1.0 × 10−10	<1.0 × 10−10
	Vancomycin	<1.6 × 10−10	<1.6 × 10−10	<1.6 × 10−10
	Linezolid	∼4 × 10−6	<2 × 10−10	<2 × 10−10
	Quinupristin-dalfopristin	<2 × 10−10	<2 × 10−10	<2 × 10−10
	Daptomycin	<2 × 10−10	<2 × 10−10	<2 × 10−10
SA504; MRSA, VISA	CG400549	<1.4 × 10−10	<1.4 × 10−10	<1.4 × 10−10
	Vancomycin	<3.7 × 10−10	<3.7 × 10−10	<3.7 × 10−10
	Linezolid	<3.7 × 10−10	<3.7 × 10−10	<3.7 × 10−10
	Quinupristin-dalfopristin	<1.6 × 10−10	<1.6 × 10−10	<1.6 × 10−10
	Daptomycin	∼4 × 10−6	∼2 × 10−8	∼3.6 × 10−9
SA481; MRSA	CG400549	∼3.0 × 10−9	∼3.0 × 10−9	∼8.0 × 10−10
	Vancomycin	<1.7 × 10−10	<1.7 × 10−10	<1.7 × 10−10
	Linezolid	∼1.7 × 10−6	<1.7 × 10−10	<1.7 × 10−10
	Quinupristin-dalfopristin	<1.2 × 10−10	<1.2 × 10−10	<1.2 × 10−10
	Daptomycin	<1.9 × 10−10	∼4.7 × 10−8	∼3.9 × 10−9
SA490; MRSA	CG400549	∼1.9 × 10−9	∼1.4 × 10−9	<1.4 × 10−10
	Vancomycin	<1.3 × 10−10	<1.3 × 10−10	<1.3 × 10−10
	Linezolid	<1.4 × 10−10	<1.4 × 10−10	<1.4 × 10−10
	Quinupristin-dalfopristin	∼1.3 × 10−10	<1.3 × 10−10	<1.3 × 10−10
	Daptomycin	∼7.6 × 10−8	∼2.6 × 10−8	∼6.4 × 10−9
SA525; MRSA	CG400549	∼5.3 × 10−8	∼7.3 × 10−9	∼2.3 × 10−9
	Vancomycin	<2.1 × 10−10	∼3.2 × 10−9	<2.1 × 10−10
	Linezolid	∼1.1 × 10−4	<2.1 × 10−10	<2.1 × 10−10
	Quinupristin-dalfopristin	∼1.1 × 10−9	<1.1 × 10−10	<1.1 × 10−10
	Daptomycin	∼2.7 × 10−8	<1.3 × 10−10	<1.3 × 10−10
SA547; MRSA	CG400549	∼1.1 × 10−8	∼2.2 × 10−9	∼1.3 × 10−9
	Vancomycin	<1.7 × 10−10	<1.7 × 10−10	<1.7 × 10−10
	Linezolid	∼6.3 × 10−6	<1.6 × 10−10	<1.6 × 10−10
	Quinupristin-dalfopristin	∼4.1 × 10−10	<1.4 × 10−10	<1.4 × 10−10
	Daptomycin	<1.9 × 10−10	<1.9 × 10−10	<1.9 × 10−10
SA540; MSSA	CG400549	∼1.9 × 10−7	∼2.5 × 10−9	∼1.3 × 10−9
	Vancomycin	<1.2 × 10−10	<1.2 × 10−10	<1.2 × 10−10
	Linezolid	<1.3 × 10−10	<1.3 × 10−10	<1.3 × 10−10
	Quinupristin-dalfopristin	∼1.2 × 10−10	∼2.4 × 10−10	<1.2 × 10−10
	Daptomycin	∼1.2 × 10−6	<1.2 × 10−10	∼4.9 × 10−9
SA543; MSSA	CG400549	∼2.2 × 10−7	∼1.2 × 10−8	∼5.2 × 10−9
	Vancomycin	∼1.3 × 10−5	∼4.7 × 10−5	∼1.4 × 10−6
	Linezolid	<1.4 × 10−10	<1.4 × 10−10	<1.4 × 10−10
	Quinupristin-dalfopristin	<1.1 × 10−10	<1.1 × 10−10	<1.1 × 10−10
	Daptomycin	<2 × 10−10	<2 × 10−10	<2 × 10−10

^aNT, not tested.