TABLE 5.
Effects of BPQ-OH and compounds 1b, 3d, and 3g on the free sterol composition of Leishmania mexicana (NR) promastigotesa
| Sterol source and sterol | Composition (mass %) after treatment with the following compound at the indicated concn:
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BPQ-OH
|
1b
|
3d
|
3g
|
|||||||||
| 0 μM | 1 μM | 3 μM | 0 μM | 0.1 μM | 0.3 μM | 0 μM | 0.1 μM | 0.3 μM | 0 μM | 0.3 μM | 1 μM | |
| Exogenous, cholesterol | 13.9 | 17.5 | 39.6 | 13.9 | 31.4 | 36.7 | 7.4 | 31.6 | 52.9 | 17.2 | 13.9 | 41.2 |
| Endogenous | ||||||||||||
| 5-Dehydro episterol | 67.3 | 65.7 | 39.6 | 67.3 | 26.4 | 18.1 | 82.7 | 53.0 | 24.2 | 70.8 | 72.7 | 43.0 |
| Episterol | 12.5 | 16.8 | 20.8 | 12.5 | 42.2 | 33.3 | 8.9 | 15.4 | 22.9 | 12.0 | 13.4 | 15.8 |
a
The sterols were extracted from cells exposed to the indicated drug concentration for 96 h; they were separated from polar lipids by silicic acid column chromatography and analyzed by quantitative capillary gas-liquid chromatography and mass spectrometry.