FIG. 2.
Antimicrobial activity of hBD-2 in the presence of human tears or NaCl solutions. Ciloxan, ciprofloxacin HCl ophthalmic solution. (A) A total of 107 CFU/ml P. aeruginosa (ATCC 27853) was incubated with 0.01% acetic acid, hBD-2 (100 μg/ml), or hBD-2 (100 μg/ml) and tears (17.5%, 35%, or 70% [vol/vol]) at 37°C for 2 h. Mean data, expressed as the percentage of bacteria killed by hBD-2 in tears obtained from five subjects, are shown. (B) A total of 107 CFU/ml P. aeruginosa (ATCC 27853) was incubated with 0.01% acetic acid, hBD-2 (100 μg/ml), or hBD-2 (100 μg/ml) and NaCl solutions at 37°C for 2 h. Average data from three experiments, expressed as the percentage of bacteria killed by hBD-2 in the presence of NaCl, are shown.