FIG. 5.

DNA binding activity of mPE. A single-stranded GC-rich oligonucleotide (ssDNA) was incubated at 37°C in the absence or presence of mPE at a molar ratio (mPE to DNA) of 1:1, 0.1:1, or 0.01:1, followed by agarose gel electrophoresis. The gel was visualized after ethidium bromide staining and UV irradiation. Double-stranded DNA was created by annealing of the single-stranded oligonucleotide at 65°C in isotonic saline buffer. The double-stranded oligonucleotide was incubated with mPE at a molar ratio of 1:1 (1:1ds) or in buffer alone (ds). The results shown are representative of three experiments. M, molecular marker.