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. Author manuscript; available in PMC: 2007 Dec 21.
Published in final edited form as: FEBS Lett. 2007 Nov 13;581(29):5561–5565. doi: 10.1016/j.febslet.2007.10.062

Figure 3.

Figure 3

Summary of NEM modifications in each of the HPLC peaks. Vertical red lines indicate trypsin cleavage sites and Cys residues with a blue background identify NEM modifications. Peak b contains two modifications thus identifying the Cys111-Cys132 disulfide. Peak c contains two additional modifications demonstrating linkage between Cys116-Cys123, and so forth. The resulting disulfide linkage map is shown below the sequences. The thin lines are consistent with disulfides identified in the NMR structure; the thick lines are newly identified by chemical mapping here.