Figure 4.
Structure of ASIP-YY showing the complete disulfide map. The yellow colored disulfide bonds are consistent with those identified in the NMR structure; the green bonds are identified here by chemical mapping here. The disulfide bond from Cys107 to Cys125 threads from the front to the back of the protein and demonstrates unequivocally that ASIP is a member of the inhibitor cystine knot family.