Figure 10.

Effect of cytochalasin B and phalloidin on the CT response to HCl. (A) The CT responses to 20 mM HCl + 10 mM KCl (R+HCl) were recorded relative to the rinse solution, R (10 mM KCl). CT responses were also recorded with both rinse (R+M) and the HCl solution (R+M+HCl) containing, in addition, 500 mM mannitol (M). CT responses were recorded under control conditions (Control), after topical lingual application of 20 μM cytochalasin B for 20 min (Post-cyto B), and after topical lingual application of 1.2 mM phalloidin for 30 min (Post-cyto B and phalloidin). (B) Summary of the effect of cytochalasin B and phalloidin on the CT response to HCl. The relative peak phasic responses (a–b, c–d, e–f, g–h, i–j, and k–l) from three animals were normalized to tonic 300 mM NH₄Cl in each individual animal and presented as mean ± SEM for control (black bar), post-cytochalasin (white bar), and post-cytochalasin B and phalloidin treatment (hatched bar). *, P < 0.05; **, P < 0.01; ***, P < 0.001.