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. 2006 Jan;127(1):35–50. doi: 10.1085/jgp.200509317

Figure 9.

Figure 9.

Voltage and Mg2+-dependent activation of WT, 5D5N + D367A + E399N, and 5D5N + D362A:D367A + E399N mutant channels. (A) Current traces of WT mslo1 channels (left), 5D5N + D367A + E399N mutant channels (middle), and 5D5N + D362A:D367A + E399N mutant channels (right) recorded at indicated [Mg2+]i. Testing potentials were −200 to 140 mV with 20-mV increments. The holding and repolarizing potentials were −80 and −120 mV, respectively. (B) Mean G-V relations of 5D5N + D367A + E399N (left) and 5D5N + D362A:D367A + E399N mutant channels (right) in the presence of 0–100 mM [Mg2+]i and fittings with the hypothesis 3 model. The symbols represent [Mg2+]i (in mM) at 0 (open circle), 0.1 (closed circle), 1 (open triangle), 10 (closed triangle), 30 (open diamond), and 100 (closed diamond). The data were fitted (solid lines) with Eq. 4. The parameters were fixed with the numbers in Table III, except L0, which were set free. In the fits, L0 for 5D5N + D367A + E399N and 5D5N + D362A:D367A + E399N is 3516 and 4908, respectively.