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. 2006 Feb;127(2):145–158. doi: 10.1085/jgp.200509439

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — Western blot analysis of some selected mutants, compared with wt SGLT1, detected with an anti-myc antibody. Extracts enriched in plasma membrane were purified from noninjected oocytes (A), wt SGLT1–injected oocytes (B), and mutants C345A (C), C351A (D), C355A (E), and C361A (F) and double mutant C_522,560A (G) and were loaded onto a polyacrylamide gel (equivalent to membranes from 20 oocytes), and protein equivalencies were confirmed by Ponceau Red staining. Molecular standard masses are indicated on the left (in kD) (New England Biolabs).