. 2006 Feb;127(2):145–158. doi: 10.1085/jgp.200509439

TABLE I.

Rate Constant of the Four-state Kinetic Model Used for the Calculations of Time Constants and Transferred Charges of wt SGLT1 and Mutants

	k₁₂₀ ^a	k₂₁₀	k₂₃₀	k₃₂₀	k₃₄₀	k₄₃₀	k₄₁₀ ^b	V_1/2 ^c
	s ⁻¹	s ⁻¹	s ⁻¹	s ⁻¹	M ⁻¹ s ⁻¹	s ⁻¹	s ⁻¹	mV
wt SGLT1	790	370	100	180	8000	2300	10	−39
wt ± DTT, C255A, C511A, and C_255,511A	790	370	230	180	8000	2300	10	−20

The constant k₁₂₀ (as well as other k_ij0) represents the value of k₁₂ (and k_ij) at 0 mV.

The constant k₁₄₀ (in s⁻¹M⁻¹) was calculated in order to respect the micro reversibility as described in Appendix A.

The V_1/2 was obtained from a fit of a Boltzmann relation to the Q-V curve calculated with the model as described in Appendix B. The experimental values are cited in the text.