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. 2006 Feb;127(2):171–182. doi: 10.1085/jgp.200509456

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — Expression of IP3Rs in SolC1(−) and SolD(+) myotubes. RT-PCR–based analysis of IP3R-1 (A), IP3R-2 (B), and IP3R-3 (C) mRNA for 40 cycles. S6 ribosomal mRNA was used as an internal standard. Relative mRNA expression levels of IP3R-1, IP3R-2, and IP3R-3 was compared with S6 ribosomal mRNA expression. Expression of IP3R isoforms in SolC1(−) myotubes were compared with normalized SolD(+) one. Each bar graph corresponds to the mean value ± SEM obtained for n = 4 (n number of experiments). ns, not significantly different. ***, P < 0,001; **, 0.001 ≤ P < 0.01; *, 0.01 ≤ P < 0.05 (Student's unpaired t test).