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. 2006 Mar;127(3):341–352. doi: 10.1085/jgp.200509418

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Copyright © 2006, The Rockefeller University Press

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Figure 1. — (A) Functional expression of Na,K-ATPase cysteine mutants. The K⁺-induced current (I_K+, gray bars) and ouabain-sensitive current (I_ouab, black bars) are shown for oocytes injected with the cRNA of the β1 and α1 subunits of the WT and cysteine mutants of residue positions 314–326 of the α1 subunit. Currents were recorded at −50 mV. Positive current values indicate an outward current. There were between 4 and 15 measurements in each group. (B) Effect of MTSET. The current measured after exposure to 250 μM MTSET for 2 min is reported after normalizing for the current measured before exposure to MTSET. There were between five and eight measurements in each case. The four mutants showing major modifications after MTSET treatment, and which were studied in greater detail, are highlighted.