TABLE I.
Shift of the SV Activation Potential by Neomycin
| [neomycin] (μM) |
V′1/2
(mV ± SEM) |
z′ (±SEM) |
V″1/2
(mV ± SEM) |
z″ (±SEM) |
N |
|---|---|---|---|---|---|
| First Boltzmann | Second Boltzmann | ||||
| 0 | +45 ± 1 | 2.5 ± 0.1 | – | – | 9 |
| 0.5 | +47 ± 2 | 2.3 ± 0.1 | – | – | 3 |
| 2 | +41 ± 2 | 2.4 ± 0.2 | – | – | 4 |
| 10 | +35 ± 2 | 2.2 ± 0.2 | – | – | 5 |
| 50 | +33 ± 3 | 2.0 ± 0.2 | – | – | 5 |
| 200 | +32 ± 3 | 1.7 ± 0.6 | −34 ± 15 | 4.0 ± 3.8 | 4 |
| 1000 | +32 ± 5 | 1.9 ± 0.5 | −44 ± 6 | 5.4 ± 2.6 | 3 |
Boltzmann analysis of instantaneous SV tail currents in the presence of different neomycin concentrations. Data from N experiments were averaged and subjected to a weighted best fit with one or, at neomycin concentrations >200 μM, two Boltzmann functions. Columns show the neomycin concentration, the voltage of half activation V1/2, and the apparent gating charge z.