. 2006 Mar;127(3):253–265. doi: 10.1085/jgp.200509423

TABLE II.

Gel Shift Analyses for Na_v from Neonatal and Adult Cardiomyocytes under Conditions of Full and Reduced Glycosylation/Sialylation

Myocyte type	Untreated (n)	PNGase-F (n)	Neuraminidase (n)
Neonatal ventricle	246.5 ± 0.8 (19)	231.6 ± 1.9 (6)^a	241.2 ± 0.7 (13)^a
Adult ventricle	251.8 ± 1.2 (18)^a	231.3 ± 2.0 (6)^a	242.0 ± 1.1 (12)^a
Neonatal atria	252.2 ± 0.5 (12)^a	230.2 ± 1.1 (4)^a	243.1 ± 0.6 (8)^a
Adult atria	251.4 ± 0.4 (11)^a	230.7 ± 1.2 (4)^a	243.3 ± 0.5 (7)^a

Data list the mean apparent molecular weight (M_r; in kD) ± SEM measured for neonatal and adult cardiomyocyte Na_v α subunits. The number of samples, n, for each is listed in parentheses. For the untreated data, significance was determined using a two-tailed Student's t test comparing the α subunit M_r from neonatal atria and adult atria and ventricles to the α subunit M_r from neonatal ventricles. The same test for significance was used comparing treated homogenates to untreated controls.

Highly significant (P < 0.01).