TABLE I.
Permeability Ratios of Various Anions in the WT CLC-0, K149L, and K149R Mutants
| SCN− | Cl− | Br− | NO3 − | I− | F− | ||
|---|---|---|---|---|---|---|---|
| WT (n = 3) | Erev (mV) | 7.1 ± 0.4 | 0.1 ± 0.0 | −9.8 ± 0.3 | −17.2 ± 0.6 | −38.9 ± 1.1 | −77.0 ± 3.5 |
| PX/PCl | 1.33 ± 0.02 | – | 0.68 ± 0.01 | 0.51 ± 0.01 | 0.22 ± 0.01 | 0.05 ± 0.01 | |
| K149L (n = 4) | Erev (mV) | 20.6 ± 0.6 | 0.4 ± 0.1 | −1.3 ± 0.1 | −2.6 ± 0.1 | −19.6 ± 2.4 | −39.8 ± 2.9 |
| PX/PCL | 2.25 ± 0.05 | – | 0.95 ± 0.00 | 0.90 ± 0.01 | 0.47 ± 0.04 | 0.21 ± 0.02 | |
| K149R (n = 4) | Erev (mV) | 11.5 ± 2.0 | 0.4 ± 0.2 | −3.6 ± 0.7 | −4.8 ± 0.6 | −16.5 ± 1.9 | −44.6 ± 2.6 |
| PX/PCl | 1.59 ± 0.12 | – | 0.87 ± 0.02 | 0.83 ± 0.02 | 0.53 ± 0.04 | 0.18 ± 0.02 |