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. 2006 May;127(5):467–480. doi: 10.1085/jgp.200509467

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Copyright © 2006, The Rockefeller University Press

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Figure 6. — Residue 169C is not protected by spermine or CGC-11179 occupancy of the Kir6.2 pore. Patches expressing Kir6.2 [N160D][C166S][M169C] were preblocked by voltage steps to +50 mV in either (A) 10 μM spermine or (B) CGC-11179 and exposed to a polyamine-free solution containing 100 μM MTSEA, as described in Figs. 4 and 5. (C) Modification of channels preblocked with either CGC-11179 (red symbols, τ = 3.3 ± 0.3 s) or spermine (green symbols, τ = 2.7 ± 0.2 s) in multiple patches. The time course of unprotected modification of M169C is indicated by the blue line (τ = 2.4 ± 0.3 s). Pore occupancy by either polyamine does not significantly alter the rate of cysteine modification at 169C.