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. 2006 May;127(5):539–555. doi: 10.1085/jgp.200609496

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Copyright © 2006, The Rockefeller University Press

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Figure 2. — Time course of modification of Cys-448 by MTS reagents. (A) Original voltage (top) and current (bottom) trace acquired from an oocyte expressing S448C and repeatedly exposed to 25 μM MTSEA for successive 1-min intervals (white bars). During MTSES exposure, the membrane potential was held at −90 mV. After washout of MTSES, the membrane potential was changed to −50 mV and 1 mM P_i was applied (gray bars) to record the P_i-induced current. (B–D) , as a function of cumulative exposure time in MTSES (B), MTSEA (C), and MTSEA in the absence of Na⁺ (ND0 superfusate) (D). Abscissa is the cumulative exposure time (t) at the indicated incubation holding potential. In each panel, (at V _h = −50 mV) after each MTS exposure was normalized to at t = 0 and fitted with Eq. 3 (solid line) to obtain the effective second-order rate constant (see Table I).

Inline graphic — Time course of modification of Cys-448 by MTS reagents. (A) Original voltage (top) and current (bottom) trace acquired from an oocyte expressing S448C and repeatedly exposed to 25 μM MTSEA for successive 1-min intervals (white bars). During MTSES exposure, the membrane potential was held at −90 mV. After washout of MTSES, the membrane potential was changed to −50 mV and 1 mM P_i was applied (gray bars) to record the P_i-induced current. (B–D) , as a function of cumulative exposure time in MTSES (B), MTSEA (C), and MTSEA in the absence of Na⁺ (ND0 superfusate) (D). Abscissa is the cumulative exposure time (t) at the indicated incubation holding potential. In each panel, (at V _h = −50 mV) after each MTS exposure was normalized to at t = 0 and fitted with Eq. 3 (solid line) to obtain the effective second-order rate constant (see Table I).