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. 2006 May;127(5):539–555. doi: 10.1085/jgp.200609496

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — P_i dependency of the voltage-dependent fluorescence in oocytes expressing S448C. (A) Original fluorescence trace recorded in ND100 (top) and ND100 + 1 mM P_i (bottom) solutions from an oocyte labeled with MTS-TMR. The membrane voltage was stepped from V _h = −60 mV to voltages in the range −200 to +80 mV in 40-mV increments, as indicated. Data were lowpass filtered at 500 Hz. (B) P_i dependency of the voltage-dependent fluorescence (ΔF). Steady-state fluorescence at different membrane potentials was acquired for each P_i concentration indicated in the figure. Data points are joined for visualization only. (C) Data in B were replotted as a function of the P_i concentration and fitted with Eq. 4 (solid lines). (D) , as reported by the fit of Eq. 4 to the data in C (H constrained to 1).

Inline graphic — P_i dependency of the voltage-dependent fluorescence in oocytes expressing S448C. (A) Original fluorescence trace recorded in ND100 (top) and ND100 + 1 mM P_i (bottom) solutions from an oocyte labeled with MTS-TMR. The membrane voltage was stepped from V _h = −60 mV to voltages in the range −200 to +80 mV in 40-mV increments, as indicated. Data were lowpass filtered at 500 Hz. (B) P_i dependency of the voltage-dependent fluorescence (ΔF). Steady-state fluorescence at different membrane potentials was acquired for each P_i concentration indicated in the figure. Data points are joined for visualization only. (C) Data in B were replotted as a function of the P_i concentration and fitted with Eq. 4 (solid lines). (D) , as reported by the fit of Eq. 4 to the data in C (H constrained to 1).