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Effective Second Order Rate Constants (k) for MTS Modification of Cys-448
| V h (mV) | MTSEA (ND100) | MTSEA (ND0) | MTSES (ND100) |
|---|---|---|---|
| 0 | 2.9 ± 0.3 | 1.0 ± 0.2 | 0.20 ± 0.04 |
| −90 | 7.1 ± 0.4 | 2.6 ± 0.4 | 0.55 ± 0.08 |
k (in mM−1s−1) was obtained by fitting time-dependent modification data using Eq. 3. Values are mean ± SEM of fit to pooled, normalized data. V h is holding potential during labeling with given reagent. Superfusion conditions are indicated in parentheses.
