TABLE I.
Cl − and HCO3 − Fluxes and Stoichiometry of Cl − /HCO3 − Exchange by AE1, slc26a3, and slc26a6 Transporters
| AE1a
b
|
slc26a3a
b
c
|
slc26a6a
b
d
|
||||||
|---|---|---|---|---|---|---|---|---|
| Cl− | HCO3 − | Ratio | Cl− | HCO3 − | Ratio | Cl− | HCO3 − | Ratio |
| 3.8 | 3.6 | 1.06 | 8.4 | 4.2 | 2.00 | 0.9 | 1.81 | 0.5 |
| 3.1 | 3.3 | 0.94 | 8.3 | 4.6 | 1.80 | 0.75 | 1.35 | 0.56 |
| 2.9 | 3.1 | 0.96 | 7.5 | 3.8 | 1.97 | 0.75 | 1.35 | 0.56 |
| 3.0 | 3.3 | 0.92 | 6.5 | 3.2 | 2.03 | 1.05 | 1.80 | 0.58 |
| 3.1 | 3.2 | 0.98 | 7.1 | 3.5 | 2.03 | 1.20 | 2.11 | 0.57 |
| 2.3 | 2.4 | 0.97 | 6.2 | 3.5 | 1.77 | 0.75 | 1.53 | 0.49 |
| 2.5 | 2.5 | 1.00 | 5.2 | 2.7 | 1.93 | 0.90 | 1.51 | 0.60 |
| 2.8 | 3.0 | 0.93 | 6.7 | 3.7 | 1.81 | 0.60 | 1.12 | 0.54 |
| 2.9 | 2.9 | 0.98 | 6.6 | 3.3 | 1.98 | 1.00 | 1.80 | 0.56 |
| 2.9 | 3.2 | 0.92 | 5.9 | 2.8 | 2.11 | 0.75 | 1.22 | 0.61 |
| 5.2 | 2.6 | 2.00 | ||||||
| 7.6 | 3.8 | 2.00 | ||||||
| 0.96 ± 0.04e | 1.96 ± 0.03e | 0.56 ± 0.03e | ||||||
All Cl− and HCO3 − transport rates are in μM/min recorded upon the first Cl− removal of oocytes incubated in HCO3 −-buffered media (Clin −/HCO3out − exchange).
The first sets of Cl− and HCO3 − transport and the ratios for each transporter were taken from Fig. 1 B.
The second and third sets for slc26a3 were taken from Fig. 2 (A and C, respectively).
The second set for slc26a6 was taken from Fig. 8 A.
Mean ± SEM.