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. 2006 Jun;127(6):719–735. doi: 10.1085/jgp.200509438

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — Examples of experimentally obtained shut-time distributions, and calculation of t _crit values. For each histogram, the red and blue lines are the mixtures of exponential densities corresponding to the components to either side of the cut (see Materials and Methods). It can be shown that the t _crit for any given distribution (open circles) coincides with the time value at which these two exponential densities intersect (Eq. 3). The sum of the red and blue lines gives, of course, the mixture of exponential densities corresponding to all the components of the distribution (bear in mind, though, that the ordinates are plotted as the square root). The t _crit value, the total number of shuttings, and the fraction of misclassified shuttings in these example histograms are, respectively, as follows: A: 65.44 ms, 12,051, 0.008; B: 15.92 ms, 19,664, 0.124; C: 45.03 ms, 19,239, 0.012; D: 5.40 ms, 45,564, 0.007; E: 5.14 ms, 34,274, 0.008; F: 12.41 ms, 4,029, 0.022; G: 1.09 ms, 40,149, 0.009, H: 1.99 ms, 29,204, 0.004, I: 4.28 ms, 4,707, 0.054.