Figure 1.

MthK is an inwardly rectifying, Ca²⁺-gated channel. (A) Single MthK channel recordings in 0 and 5 mM intracellular Ca²⁺ to illustrate overall MthK gating. (B) Representative MthK channel bursts as a function of voltage in 0 and 5 mM intracellular Ca²⁺ at the indicated voltage. (C) Characteristic single-channel current voltage I-V curves where each data point represents the mean ± SEM from at least eight (5 mM Ca²⁺, filled circles) and three (0 mM Ca²⁺, open circles) separate bilayers. * indicates mean ± SEM from only two bilayers. All records are low-pass filtered at 2 kHz and sampled at 10 kHz unless specified otherwise.