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. 2006 Jun;127(6):659–672. doi: 10.1085/jgp.200609504

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — Currents in an eosinophil studied in perforated-patch configuration with K-containing pipette solution. Identical families of pulses (inset) were applied (A) before stimulation, (B) 5.6 min after PMA addition, (C) 2.3 min after addition of 100 nM IbTX, (D) and after addition of 3 mM ZnCl₂. Arrows indicate zero current. From a holding potential of −60 mV, 8-s pulses were applied every 20 s in 20-mV increments from −40 to +80 mV. The total inward current (leak plus electron current) was −1.8 pA in A, −8.8 pA in B, −10.8 pA in C, and −8.5 pA in D. Taking the time the family in A was recorded as 0 min, the family in B was started at 11.0 min, C at 15.1 min, and D at 24.7 min. Filter, 1 kHz, bath solution pH 7.0 TMAMeSO₃ and pipette solution KMeSO₃; both solutions contain 50 mM .

Inline graphic — Currents in an eosinophil studied in perforated-patch configuration with K-containing pipette solution. Identical families of pulses (inset) were applied (A) before stimulation, (B) 5.6 min after PMA addition, (C) 2.3 min after addition of 100 nM IbTX, (D) and after addition of 3 mM ZnCl₂. Arrows indicate zero current. From a holding potential of −60 mV, 8-s pulses were applied every 20 s in 20-mV increments from −40 to +80 mV. The total inward current (leak plus electron current) was −1.8 pA in A, −8.8 pA in B, −10.8 pA in C, and −8.5 pA in D. Taking the time the family in A was recorded as 0 min, the family in B was started at 11.0 min, C at 15.1 min, and D at 24.7 min. Filter, 1 kHz, bath solution pH 7.0 TMAMeSO₃ and pipette solution KMeSO₃; both solutions contain 50 mM .