Figure 8.

Single channels in S2 cells treated with dBest1 1C RNAi and in CHO cells expressing dbest1. (A) An inside-out patch was excised from an S2 cell treated with RNAi into 0 Ca²⁺ solution. During the break in the record, the patch was switched to solution containing 650 nM free Ca²⁺. A few single channel openings can be seen. (B) Single channel opening from A shown on a faster time base. (C) Single channels in an inside-out excised patch exposed to 650 nM Ca²⁺ solution from a cell transfected with dbest1. (D) A portion of the trace in C shown on a faster time base. (E) All points histogram of the trace in C.