TABLE I.
Primers for RNAi Synthesis
| Primer | Sequence | Product size | |
|---|---|---|---|
| CON | Forward | 5′-TCGGGGCTGTGGCTGAGGT-3′ | 785 bp |
| Reverse | 5′-TGGTGCTTCGCGTTGATGTGT-3′ | ||
| 1N | Forward | 5′-ATCCCATCGCCGTGTTTGT-3′ | 814 bp |
| Reverse | 5′-ACCTCGATCTTGGCAGTGGAC-3′ | ||
| 1C | Forward | 5′-GCAACGCCCAGTCAGGA-3′ | 793 bp |
| Reverse | 5′-TCATCGTCGAATTGGAGAAC-3′ | ||
| 1S | Forward | 5′-TGATGCCAGTGGCATTCAC-3′ | 509 bp |
| Reverse | 5′-CGCCAGGTGGAAATAGGTT-3′ | ||
| 2N | Forward | 5′-GATACGTTGGCCCTGTTCATAA-3′ | 793 bp |
| Reverse | 5′-GCTCGTCTTTCCGGTAGTGTTC-3′ | ||
| 2C | Forward | 5′-CCAGCTCGGTCCTAATG-3′ | 798 bp |
| Reverse | 5′-CCTCTCCGGTCTTTTGTT-3′ | ||
| 2S | Forward | 5′-AAACATCACCACTCTGTCGT-3′ | 503 bp |
| Reverse | 5′-TTGAGGGGGCCGAGGAT-3′ | ||
| 3N | Forward | 5′-GATCGGGATATTAAGCACTACA-3′ | 783 bp |
| Reverse | 5′-GTCCTCCTCCTTTCTCTTTTTC-3′ | ||
| 4N | Forward | 5′-TGGCCACGTACTCCTTCTTCCT-3′ | 784 bp |
| Reverse | 5′-GCTCTGTCCCCCGCTTCCT-3′ |
T7 sites (5′-CTAATACGACTCACTATAGGGAG-3′) were added to 5′ ends of both forward and reverse primers.