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. 2006 Nov;128(5):593–604. doi: 10.1085/jgp.200609648

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Copyright © 2006, The Rockefeller University Press

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Figure 8. — Summary of effects of altering PI(4,5)P₂ levels on HCN2 gating. Average shifts in V_1/2 shown in response to various agents using either inside-out patches (gray bars) or whole oocytes (open bars). From top to bottom: bath only (no treatment); 2 mM MgATP (in FV solution, 0.05% DMSO); 2 mM MgATP plus 15 μM wortmannin (in FV solution, 0.05% DMSO); 25 μg/ml poly-d-lysine; 30.3 μg/ml anti-PI(4,5)P₂ antibody (αPIP₂ Ab); 30.3 μg/ml heat-inactivated anti-PI(4,5)P₂ antibody; 30.3 μg/ml anti-granulocyte macrophage colony stimulating factor antibody (αGM-CSF Ab); V_1/2 of HCN2 coexpressed with PI(4)P 5-kinase minus V_1/2 of HCN2 expressed alone (PIP5K); V_1/2 of HCN2 after 30–40-min preincubation in 15 μM wortmannin and 0.05% DMSO minus V_1/2 after preincubation in 0.05% DMSO alone (Wortmannin); V_1/2 after 2-h preincubation in 20 μM LY294002 and 0.1% DMSO minus V_1/2 after preincubation in 0.1% DMSO alone (LY294002). Error bars show SEM. Number of experiments shown next to each bar (n).