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. 2006 Nov;128(5):535–545. doi: 10.1085/jgp.200609516

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — Mutagenesis of positively charged arginine residues causes rectification of the single channel current–voltage relationship. (A) Example single channel currents carried by Cl⁻ influx or Cl⁻ efflux atthe membrane potentials indicated. For each trace, the line to the left represents the current level when the channel is closed. (B) Mean single channel current–voltage relationships constructed from such recordings for wild type (▪), R303E (•) and R352E (◯). Mean of data from three to five patches.