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. 2006 Dec;128(6):659–669. doi: 10.1085/jgp.200609650

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Copyright © 2006, The Rockefeller University Press

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Figure 6. — Generation of astrocytic endfoot [Ca²⁺]_i increases in response to treatment with vasoactive neuropeptides. (A and B) Fluo-4 AM loaded cortical slices were superfused with either SOM or VIP by means of a Picospritzer and locally (within 30 μm of endfoot) placed micropipette, and the resultant changes in astrocytic [Ca²⁺]_i were measured with confocal microscopy. All agonist treatments were performed in the presence of 1 μM tetrodotoxin, which was included in the aCSF solution to limit neuronal activity.